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Apimondia Apimedica-Apiquality International Forum

Oct. 22-25, 2012

Zhenjiang, China

Comparative Study of Propolis and its Extract on Different Volatile Oil Constituents

Yang Qin, Zhou Juan, Huang Xinxin, Zhang Lin, Zhu Huifen, Yu Shumei, Mao Riwen

(Natural Medicine Engineering Technology Research Center by Supercritical Fluid Extraction in Jiangsu province, Zhenjiang 212009,China)

ObjectiveTo determine the amount of volatile oil in propolis glue using the following methods: solvent extraction, propolis ethanol extraction and supercritical CO2 extraction. GC-MS was used to determine both the qualitative and quantitative data.

ResultThe content of volatile oil in the propolis was in the following order: supercritical CO2 extraction > ethanol extraction > propolis glue. The content of volatile oil in the propolis extracted by supercritical CO2 was 23.45% higher than the propolis extracted with ethanol. The results of GC-MS

indicated that propolis extracted using supercritical CO2 method recorded a high content of volatile oil (54.8%) in two kinds of common volatile componential extracts which was superior to the propolis extracted with ethanol. More than 1% of the composition of volatile propolis extracted by supercritical CO2 method accounted for 64.7%. This indicated that the volatile oil composition in propolis extracted by supercritical CO2 method was better than the ones extracted with ethanol. Especially terpene; a kind of component found in the propolis extracted by supercritical CO2 was 1.5 times higher than the propolis extracted with ethanol.

ConclusionThe content of propolis extracts and its volatile oil was highest in the propolis extracted by supercritical CO2. The results indicated that the supercritical CO2 extraction method for extracting propolis and its volatile oil is better than that of ethanol extraction.

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蜂胶及其不同提取物中挥发油成分的比较研究

杨琴,周娟,黄鑫鑫,张林,朱惠芬,郁树美,毛日文

(江苏省超临界萃取天然药物工程技术研究中心,镇江 212009,中国)

【目的】用溶剂萃取蜂胶原胶、蜂胶乙醇提取物及蜂胶超临界C02提取物中的蜂胶挥发油;利用GC-MS 对蜂胶挥发油进行定性与定量分析。

【结果】蜂胶原胶及其提取物中的挥发油含量依次为蜂胶超临界C02提取 >蜂胶乙醇提取物>蜂胶原胶。蜂胶超临界C02提取物挥发油含量较之蜂胶乙醇提取物挥发油含量高

23.45%;通GC-MS分析,两种提取物共有的挥发性成分中,蜂胶超临界C02提取物中含量高的占54.8%优于蜂胶乙醇提取物;在含量>1%挥发性成分中,蜂胶超临界CO2提取物占64.7%进一步说明蜂胶超临 CO2提取物挥发性成分优于蜂胶乙醇提取物的挥发性成分。尤其是萜烯类成分,蜂胶超临界CO2提取物中 萜烯类成分含量是蜂胶乙醇提取物中萜烯类成分含量的1.5倍。

结论】蜂胶及其不同提取物中的挥发油,以 临界C02提取物中的含量最高,表明利用超临界CO2提取技术提取蜂胶挥发油要优于乙醇提取。

键词:蜂胶;超临界C02提取;乙醇提取;挥发油;GC-MS

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